mruby2 n1 (Addgene inc)
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mruby2 n1
Mruby2 N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+n1/mRuby2-N1+(Plasmid+%2354614)/us12351877-394-25-31
Average 93 stars, based on 18 article reviews
Mruby2 N1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mruby2+n1/mRuby2-N1+(Plasmid+%2354614)/us12351877-394-25-31
Average 93 stars, based on 18 article reviews
mruby2 n1 - by Bioz Stars,
2026-10
93/100 stars
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Construct:Article Title: Systems and methods for determining a treatment course of action Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion. Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1mRuby2-IRES-PuroR was generated by PCR amplification of complementary DNA (cDNA) for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene, #35680) and Article Title: IL-24 intrinsically regulates Th17 cell pathogenicity in mice Article Snippet: The sequences corresponding to full-length or nonsecretable (truncated) versions of IL-24, i.e., IL-24 and IL-24t, were cloned into mammalian expression vector pIRES-eGFP (6029-1; Clontech). .. The fusion constructs of nonsecretable IL-24 (mRuby2-N1-IL-24t; sequence: 5′-ATG CAA GAG TTC CGA TTT GGG TCT TGC CAA GTG ACA GGG GTG GTT CTC CCA GAA CTG TGG GAG GCC TTC TGG ACT GTG AAG AAC ACT GTG CAA ACT CAG GAT GAC ATC ACA AGC ATC CGG CTG TTG AAG CCG CAG GTT CTG CGG AAT GTC TCG GGT GCT GAG AGC TGT TAC CTT GCC CAC AGC CTG CTG AAG TTC TAC TTG AAC ACT GTT TTC AAG AAC TAC CAC AGC AAA ATA GCC AAA TTC AAG GTC TTG AGG TCA TTC TCC ACT CTG GCC AAC AAC TTC ATA GTC ATC ATG TCA CAA CTA CAG CCC AGT AAG GAC AAT TCC ATG CTT CCC ATT AGT GAG AGT GCA CAC CAG CGG TTT TTG CTG TTC CGC AGA GCA TTC AAA CAG TTG GAT ACA GAA GTC GCT TTG GTG AAA GCC TTT GGG GAA GTG GAC ATT CTC CTG ACC TGG ATG CAG AAA TTC TAC CAT CTC-3′) or IFN-γ (mRuby2-N1-IFN-γt; sequence: 5′-ATG CAC GGC ACA GTC ATT GAA AGC CTA GAA AGT CTG AAT AAC TAT TTT AAC TCA AGT GGC ATA GAT GTG GAA GAA AAG AGT CTC TTC TTG GAT ATC TGG AGG AAC TGG CAA AAG GAT GGT GAC ATG AAA ATC CTG CAG AGC CAG ATT ATC TCT TTC TAC CTC AGA CTC TTT GAA GTC TTG AAA GAC AAT CAG GCC ATC AGC AAC AAC ATA AGC GTC ATT GAA TCA CAC CTG ATT ACT ACC TTC TTC AGC AAC AGC AAG GCG AAA AAG GAT GCA TTC ATG AGT ATT GCC AAG TTT GAG GTC AAC AAC CCA CAG GTC CAG CGC CAA GCA TTC AAT GAG CTC ATC CGA GTG GTC CAC CAG CTG TTG CCG GAA TCC AGC CTC AGG AAG CGG AAA AGG AGT CGC TGC-3′) were generated by subcloning into Article Title: Unintended perturbation of protein function using GFP nanobodies in human cells Article Snippet: .. Briefly, EGFP was removed from the original RUSH construct Str-KDEL-SBP-EGFP-E-Cadherin ( ) by amplifying the plasmid from either side of EGFP with the following primers: forward: 5′-GGACGAGCTGTACAAGGGccggCCAgactgggtc-3′ and reverse: CGCCCTTAGACACCATACCtgcaggTGGTTCACGTTG-3′. mRuby2 was amplified using Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of complementary DNA (cDNA) for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene, #35680) and Generated:Article Title: Systems and methods for determining a treatment course of action Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion. Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1mRuby2-IRES-PuroR was generated by PCR amplification of complementary DNA (cDNA) for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene, #35680) and Article Title: IL-24 intrinsically regulates Th17 cell pathogenicity in mice Article Snippet: The sequences corresponding to full-length or nonsecretable (truncated) versions of IL-24, i.e., IL-24 and IL-24t, were cloned into mammalian expression vector pIRES-eGFP (6029-1; Clontech). .. The fusion constructs of nonsecretable IL-24 (mRuby2-N1-IL-24t; sequence: 5′-ATG CAA GAG TTC CGA TTT GGG TCT TGC CAA GTG ACA GGG GTG GTT CTC CCA GAA CTG TGG GAG GCC TTC TGG ACT GTG AAG AAC ACT GTG CAA ACT CAG GAT GAC ATC ACA AGC ATC CGG CTG TTG AAG CCG CAG GTT CTG CGG AAT GTC TCG GGT GCT GAG AGC TGT TAC CTT GCC CAC AGC CTG CTG AAG TTC TAC TTG AAC ACT GTT TTC AAG AAC TAC CAC AGC AAA ATA GCC AAA TTC AAG GTC TTG AGG TCA TTC TCC ACT CTG GCC AAC AAC TTC ATA GTC ATC ATG TCA CAA CTA CAG CCC AGT AAG GAC AAT TCC ATG CTT CCC ATT AGT GAG AGT GCA CAC CAG CGG TTT TTG CTG TTC CGC AGA GCA TTC AAA CAG TTG GAT ACA GAA GTC GCT TTG GTG AAA GCC TTT GGG GAA GTG GAC ATT CTC CTG ACC TGG ATG CAG AAA TTC TAC CAT CTC-3′) or IFN-γ (mRuby2-N1-IFN-γt; sequence: 5′-ATG CAC GGC ACA GTC ATT GAA AGC CTA GAA AGT CTG AAT AAC TAT TTT AAC TCA AGT GGC ATA GAT GTG GAA GAA AAG AGT CTC TTC TTG GAT ATC TGG AGG AAC TGG CAA AAG GAT GGT GAC ATG AAA ATC CTG CAG AGC CAG ATT ATC TCT TTC TAC CTC AGA CTC TTT GAA GTC TTG AAA GAC AAT CAG GCC ATC AGC AAC AAC ATA AGC GTC ATT GAA TCA CAC CTG ATT ACT ACC TTC TTC AGC AAC AGC AAG GCG AAA AAG GAT GCA TTC ATG AGT ATT GCC AAG TTT GAG GTC AAC AAC CCA CAG GTC CAG CGC CAA GCA TTC AAT GAG CTC ATC CGA GTG GTC CAC CAG CTG TTG CCG GAA TCC AGC CTC AGG AAG CGG AAA AGG AGT CGC TGC-3′) were generated by subcloning into Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of complementary DNA (cDNA) for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene, #35680) and Polymerase Chain Reaction:Article Title: Systems and methods for determining a treatment course of action Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion. Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1mRuby2-IRES-PuroR was generated by PCR amplification of complementary DNA (cDNA) for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene, #35680) and Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of complementary DNA (cDNA) for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene, #35680) and Article Title: RSU-1 interaction with prohibitin-2 links cell–extracellular matrix detachment to downregulation of ERK signaling Article Snippet: .. The ORFs of RSU1 and PHB2, respectively, were amplified by PCR and subloned into mClover-N1 (Addgene, no. 54538) and Article Title: RSU-1 interaction with prohibitin-2 links cell–extracellular matrix detachment to downregulation of ERK signaling Article Snippet: .. The ORFs of RSU1 and PHB2, respectively, were amplified by PCR and subloned into mClover-N1 (Addgene, no. 54538) and Amplification:Article Title: Systems and methods for determining a treatment course of action Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of cDNA for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene #35680) and Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion. Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1mRuby2-IRES-PuroR was generated by PCR amplification of complementary DNA (cDNA) for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene, #35680) and Article Title: Unintended perturbation of protein function using GFP nanobodies in human cells Article Snippet: .. Briefly, EGFP was removed from the original RUSH construct Str-KDEL-SBP-EGFP-E-Cadherin ( ) by amplifying the plasmid from either side of EGFP with the following primers: forward: 5′-GGACGAGCTGTACAAGGGccggCCAgactgggtc-3′ and reverse: CGCCCTTAGACACCATACCtgcaggTGGTTCACGTTG-3′. mRuby2 was amplified using Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion Article Snippet: .. Lentiviral construct pLenti CMV-MRLC1-mRuby2-IRES-PuroR was generated by PCR amplification of complementary DNA (cDNA) for Homo sapiens MRLC1 from eGFP-MRLC (a gift from Tom Egelhoff, Addgene, #35680) and Article Title: RSU-1 interaction with prohibitin-2 links cell–extracellular matrix detachment to downregulation of ERK signaling Article Snippet: .. The ORFs of RSU1 and PHB2, respectively, were amplified by PCR and subloned into mClover-N1 (Addgene, no. 54538) and Article Title: RSU-1 interaction with prohibitin-2 links cell–extracellular matrix detachment to downregulation of ERK signaling Article Snippet: .. The ORFs of RSU1 and PHB2, respectively, were amplified by PCR and subloned into mClover-N1 (Addgene, no. 54538) and Sequencing:Article Title: IL-24 intrinsically regulates Th17 cell pathogenicity in mice Article Snippet: The sequences corresponding to full-length or nonsecretable (truncated) versions of IL-24, i.e., IL-24 and IL-24t, were cloned into mammalian expression vector pIRES-eGFP (6029-1; Clontech). .. The fusion constructs of nonsecretable IL-24 (mRuby2-N1-IL-24t; sequence: 5′-ATG CAA GAG TTC CGA TTT GGG TCT TGC CAA GTG ACA GGG GTG GTT CTC CCA GAA CTG TGG GAG GCC TTC TGG ACT GTG AAG AAC ACT GTG CAA ACT CAG GAT GAC ATC ACA AGC ATC CGG CTG TTG AAG CCG CAG GTT CTG CGG AAT GTC TCG GGT GCT GAG AGC TGT TAC CTT GCC CAC AGC CTG CTG AAG TTC TAC TTG AAC ACT GTT TTC AAG AAC TAC CAC AGC AAA ATA GCC AAA TTC AAG GTC TTG AGG TCA TTC TCC ACT CTG GCC AAC AAC TTC ATA GTC ATC ATG TCA CAA CTA CAG CCC AGT AAG GAC AAT TCC ATG CTT CCC ATT AGT GAG AGT GCA CAC CAG CGG TTT TTG CTG TTC CGC AGA GCA TTC AAA CAG TTG GAT ACA GAA GTC GCT TTG GTG AAA GCC TTT GGG GAA GTG GAC ATT CTC CTG ACC TGG ATG CAG AAA TTC TAC CAT CTC-3′) or IFN-γ (mRuby2-N1-IFN-γt; sequence: 5′-ATG CAC GGC ACA GTC ATT GAA AGC CTA GAA AGT CTG AAT AAC TAT TTT AAC TCA AGT GGC ATA GAT GTG GAA GAA AAG AGT CTC TTC TTG GAT ATC TGG AGG AAC TGG CAA AAG GAT GGT GAC ATG AAA ATC CTG CAG AGC CAG ATT ATC TCT TTC TAC CTC AGA CTC TTT GAA GTC TTG AAA GAC AAT CAG GCC ATC AGC AAC AAC ATA AGC GTC ATT GAA TCA CAC CTG ATT ACT ACC TTC TTC AGC AAC AGC AAG GCG AAA AAG GAT GCA TTC ATG AGT ATT GCC AAG TTT GAG GTC AAC AAC CCA CAG GTC CAG CGC CAA GCA TTC AAT GAG CTC ATC CGA GTG GTC CAC CAG CTG TTG CCG GAA TCC AGC CTC AGG AAG CGG AAA AGG AGT CGC TGC-3′) were generated by subcloning into Countercurrent Chromatography:Article Title: IL-24 intrinsically regulates Th17 cell pathogenicity in mice Article Snippet: The sequences corresponding to full-length or nonsecretable (truncated) versions of IL-24, i.e., IL-24 and IL-24t, were cloned into mammalian expression vector pIRES-eGFP (6029-1; Clontech). .. The fusion constructs of nonsecretable IL-24 (mRuby2-N1-IL-24t; sequence: 5′-ATG CAA GAG TTC CGA TTT GGG TCT TGC CAA GTG ACA GGG GTG GTT CTC CCA GAA CTG TGG GAG GCC TTC TGG ACT GTG AAG AAC ACT GTG CAA ACT CAG GAT GAC ATC ACA AGC ATC CGG CTG TTG AAG CCG CAG GTT CTG CGG AAT GTC TCG GGT GCT GAG AGC TGT TAC CTT GCC CAC AGC CTG CTG AAG TTC TAC TTG AAC ACT GTT TTC AAG AAC TAC CAC AGC AAA ATA GCC AAA TTC AAG GTC TTG AGG TCA TTC TCC ACT CTG GCC AAC AAC TTC ATA GTC ATC ATG TCA CAA CTA CAG CCC AGT AAG GAC AAT TCC ATG CTT CCC ATT AGT GAG AGT GCA CAC CAG CGG TTT TTG CTG TTC CGC AGA GCA TTC AAA CAG TTG GAT ACA GAA GTC GCT TTG GTG AAA GCC TTT GGG GAA GTG GAC ATT CTC CTG ACC TGG ATG CAG AAA TTC TAC CAT CTC-3′) or IFN-γ (mRuby2-N1-IFN-γt; sequence: 5′-ATG CAC GGC ACA GTC ATT GAA AGC CTA GAA AGT CTG AAT AAC TAT TTT AAC TCA AGT GGC ATA GAT GTG GAA GAA AAG AGT CTC TTC TTG GAT ATC TGG AGG AAC TGG CAA AAG GAT GGT GAC ATG AAA ATC CTG CAG AGC CAG ATT ATC TCT TTC TAC CTC AGA CTC TTT GAA GTC TTG AAA GAC AAT CAG GCC ATC AGC AAC AAC ATA AGC GTC ATT GAA TCA CAC CTG ATT ACT ACC TTC TTC AGC AAC AGC AAG GCG AAA AAG GAT GCA TTC ATG AGT ATT GCC AAG TTT GAG GTC AAC AAC CCA CAG GTC CAG CGC CAA GCA TTC AAT GAG CTC ATC CGA GTG GTC CAC CAG CTG TTG CCG GAA TCC AGC CTC AGG AAG CGG AAA AGG AGT CGC TGC-3′) were generated by subcloning into Subcloning:Article Title: IL-24 intrinsically regulates Th17 cell pathogenicity in mice Article Snippet: The sequences corresponding to full-length or nonsecretable (truncated) versions of IL-24, i.e., IL-24 and IL-24t, were cloned into mammalian expression vector pIRES-eGFP (6029-1; Clontech). .. The fusion constructs of nonsecretable IL-24 (mRuby2-N1-IL-24t; sequence: 5′-ATG CAA GAG TTC CGA TTT GGG TCT TGC CAA GTG ACA GGG GTG GTT CTC CCA GAA CTG TGG GAG GCC TTC TGG ACT GTG AAG AAC ACT GTG CAA ACT CAG GAT GAC ATC ACA AGC ATC CGG CTG TTG AAG CCG CAG GTT CTG CGG AAT GTC TCG GGT GCT GAG AGC TGT TAC CTT GCC CAC AGC CTG CTG AAG TTC TAC TTG AAC ACT GTT TTC AAG AAC TAC CAC AGC AAA ATA GCC AAA TTC AAG GTC TTG AGG TCA TTC TCC ACT CTG GCC AAC AAC TTC ATA GTC ATC ATG TCA CAA CTA CAG CCC AGT AAG GAC AAT TCC ATG CTT CCC ATT AGT GAG AGT GCA CAC CAG CGG TTT TTG CTG TTC CGC AGA GCA TTC AAA CAG TTG GAT ACA GAA GTC GCT TTG GTG AAA GCC TTT GGG GAA GTG GAC ATT CTC CTG ACC TGG ATG CAG AAA TTC TAC CAT CTC-3′) or IFN-γ (mRuby2-N1-IFN-γt; sequence: 5′-ATG CAC GGC ACA GTC ATT GAA AGC CTA GAA AGT CTG AAT AAC TAT TTT AAC TCA AGT GGC ATA GAT GTG GAA GAA AAG AGT CTC TTC TTG GAT ATC TGG AGG AAC TGG CAA AAG GAT GGT GAC ATG AAA ATC CTG CAG AGC CAG ATT ATC TCT TTC TAC CTC AGA CTC TTT GAA GTC TTG AAA GAC AAT CAG GCC ATC AGC AAC AAC ATA AGC GTC ATT GAA TCA CAC CTG ATT ACT ACC TTC TTC AGC AAC AGC AAG GCG AAA AAG GAT GCA TTC ATG AGT ATT GCC AAG TTT GAG GTC AAC AAC CCA CAG GTC CAG CGC CAA GCA TTC AAT GAG CTC ATC CGA GTG GTC CAC CAG CTG TTG CCG GAA TCC AGC CTC AGG AAG CGG AAA AGG AGT CGC TGC-3′) were generated by subcloning into Plasmid Preparation:Article Title: Unintended perturbation of protein function using GFP nanobodies in human cells Article Snippet: .. Briefly, EGFP was removed from the original RUSH construct Str-KDEL-SBP-EGFP-E-Cadherin ( ) by amplifying the plasmid from either side of EGFP with the following primers: forward: 5′-GGACGAGCTGTACAAGGGccggCCAgactgggtc-3′ and reverse: CGCCCTTAGACACCATACCtgcaggTGGTTCACGTTG-3′. mRuby2 was amplified using |
